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KMID : 1007520000090010014
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Food Science and Biotechnology
2000 Volume.9 No. 1 p.14 ~ p.20
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Antimicrobial Low Density Polyethylene Film Coated with Bacteriocins in Binder Medium
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Kim, Young Min
Lee, Sang Baek/LEE, DONG SUN/An, Duck Soon/Paik, Hyung Dong
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Abstract
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Three types of bacteriocins (nisin, lacticin BH5, and lacticin NK24) were coated onto low density polyethylene (LDPE) film by being incorporated in a binder medium. A suitable binder medium was selected based on the stability upon contact with water. Three prospective mediums were applied, of which the polyamide resin medium showed good stability and adhesion. Coating rod was used to apply the medium mixed with bacteriocin to LDPE. The coated films were evaluated on antimicrobial activity and bacteriocin migration. The coated LDPE showed antimicrobial activity against the microbial strain, Micrococcus flavus ATCC 10240, on the agar plate medium, which was stable at 30℃ for 5 months. Addition of skim milk in the medium and increase of coating thickness did not enhance the antimicrobial activity, while application of higher concentration bacteriocin increased the activity. The migration from bacteriocins reached equilibrium in three days, but its level was too low to show wider spectrum against several bacterial strains. When the bacteriocin-coated films were exposed to phosphate buffer solution containing strains of M. flavus ATCC 10240 and Listeria monocytogenes ATCC 15313, microbial growth of both strains was significantly suppressed.
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